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Biology: Molecular Biology & DNA

20 cards|
6 easy10 medium4 hard
biologymolecular biologyDNAgene expression

DNA structure, replication, gene expression, and biotechnology techniques.

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Flashcards in This Deck

1
easy

What are the three essential components of a DNA nucleotide?

A deoxyribose sugar, a phosphate group, and a nitrogenous base (Adenine, Thymine, Cytosine, or Guanine).

2
easy

Which nitrogenous bases pair together in DNA, and how many hydrogen bonds connect each pair?

Adenine pairs with Thymine via two hydrogen bonds; Cytosine pairs with Guanine via three hydrogen bonds.

3
easy

What is the primary function of the Polymerase Chain Reaction (PCR)?

To rapidly amplify specific sequences of DNA into millions of copies through repeated cycles of heating and cooling.

4
easy

In which chemical direction is a new DNA strand always synthesized by DNA polymerase?

In the 5' to 3' direction, as nucleotides are added to the 3' hydroxyl (-OH) group.

5
easy

What is the role of tRNA during the process of translation?

tRNA molecules carry specific amino acids to the ribosome and match them to mRNA codons using their complementary anticodons.

6
easy

What physical property of DNA allows it to be separated by size during gel electrophoresis?

DNA is negatively charged due to its phosphate backbone, causing it to migrate toward the positive anode through a porous matrix.

7
medium

Why is DNA replication described as 'semi-discontinuous'?

The leading strand is synthesized continuously toward the replication fork, while the lagging strand is synthesized in short Okazaki fragments away from the fork.

8
medium

What is the function of DNA Ligase in DNA replication and repair?

It catalyzes the formation of a phosphodiester bond to seal nicks between DNA fragments, such as joining Okazaki fragments.

9
medium

How does RNA polymerase identify the correct starting point for transcription on a DNA template?

It recognizes and binds to specific DNA sequences called promoters, which are located upstream of the gene's coding region.

10
medium

What is the significance of the 'wobble' position in the genetic code?

It allows a single tRNA to recognize multiple codons by having flexible, non-standard base-pairing at the third position of the mRNA codon.

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